MK5 / MAPKAPK5 Antibody | 43-311

MK5 / MAPKAPK5 Antibody | 43-311 | Gentaur UK, US & Europe Distribution

Host: Goat

Reactivity: Human, Mouse

Homology: Expected Species Reactivity based on sequence homology: Rat, Dog, Pig, Cow

Immunogen: The immunogen for this antibody is: C-STEALDNVLPSAQ

Research Area: Obesity, Other

Tested Application: E, WB, IP, IHC

Application: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:In transfected HEK293 transiently expressing either Mouse MK5 fused to GFP or the similar Mouse MK2 fused to GFP; only the expected band was observed in the MK5 lane after EB11927 labeling. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany. Recommended concentration, 0.5-1ug/ml.Immunohistochemistry:In paraffin embedded Human Kidney shows brushborder staining in the PCT. Recommended concentration, 4-8ug/ml.Immunoprecipitation: MK5/ERK3 double knockout Mouse Embryonic Fibroblasts (MEFs) retrovirally transduced wiith Mk5/Erk3 or empty vector (GFP) were lysed from confluent plates and used for IP with 1.5ug EB11927 or EB11928. Western blots of the IP were labelled with mouse anti-MK5 (SC) or with rabbit anti-ERK3 serum (CST) . An approx 55kDa of MK5 is only precipitated from lysates of those KO MEFs that have been rescued by the ERK3/MK5 expression constructas described in PMID: 22508986. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20��C. Minimize freezing and thawing.

Concentration: 500 ug/mL

Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.

Alternate Name: MAPKAPK5, mitogen-activated protein kinase-activated protein kinase 5, MAPKAP-K5, MK-5, MK5, PRAK, MAP kinase-activated protein kinase 5, MAPK-activated protein kinase 5, MAPKAP kinase 5, MAPKAPK-5, p38-regulated/activated protein kinase

User Note: N/A

BACKGROUND: N/A