MLH1 (S467) polyclonal Antibody | BS2418

MLH1 (S467) polyclonal Antibody | BS2418 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Application: WB IF

Application Range: WB: 1:500~1:1000 IF: 1:50~1:200

Background: MLH1 is an integral part of the protein complex responsible for mismatch repair that is expressed in lymphocytes, heart, colon, breast, lung, spleen, testis, prostate, thyroid and gall bladder and is methylated in several ovarian tumors. Loss of MLH1 protein expression is associated with a mutated phenotype, microsatellite instability and a predisposition to cancer. In hereditary nonpolyposis colorectal cancer (HNPCC), an autosomal dominant inherited cancer syndrome that signifies a high risk of colorectal and various other types of cancer, the MLH1 gene exhibits a pathogenic mutation. Certain cancer cell lines, including leukemia CCRF-CEM, colon HCT 116 and KM12, and ovarian cancers SK-OV-3 and IGROV-1, show complete deficiency of MLH1, while MLH1 is expressed in 60% of melanomas, 70% of noninvasive squamous cell carcinomas and 30% of invasive squamous cell carcinomas.

Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: MLH1 (S467) polyclonal Antibody detects endogenous levels of MLH1 protein.

Molecular Weight: ~ 85 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: DNA mismatch repair protein Mlh1; MutL protein homolog 1; COCA2

Immunogen: Synthetic peptide, corresponding to amino acids 450-500 of Human MLH1.

Conjugate: Unconjugated

Modification: Unmodification

Purification & Purity: The Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Pathway: