MLL5 Antibody (N-term) [APR03921G]

MLL5 Antibody (N-term) [APR03921G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This MLL5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 93-120 amino acids from the N-terminal region of human MLL5.

Cellular Localisation: Chromosome. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Nucleus speckle. Note=Absent from the nucleolus (PubMed:14718661) . Localizes to chromosome during interphase and to centrosomes during mitosis (PubMed:23798402) . Dissociation from mitotic chromosome is likely due to histone H3 phosphorylation on 'Thr-3' and 'Thr-6' (PubMed:23798402) . [Isoform NKp44L]: Cytoplasm. Cell membrane; Peripheral membrane protein

Molecular Weight: 204965

Clone: Polyclonal

Gene Name: KMT2E

Gene ID: 55904

Function: Associates with chromatin regions downstream of transcriptional start sites of active genes and thus regulates gene transcription (PubMed:23629655, PubMed:24130829, PubMed:23798402) . Chromatin interaction is mediated via the binding to tri-methylated histone H3 at 'Lys-4' (H3K4me3) (PubMed:24130829, PubMed:23798402) . Key regulator of hematopoiesis involved in terminal myeloid differentiation and in the regulation of hematopoietic stem cell (HSCs) self-renewal by a mechanism that involves DNA methylation (By similarity) . Also acts as an important cell cycle regulator, participating in cell cycle regulatory network machinery at multiple cell cycle stages including G1/S transition, S phase progression and mitotic entry (PubMed:14718661, PubMed:18573682, PubMed:19264965, PubMed:23629655) . Recruited to E2F1 responsive promoters by HCFC1 where it stimulates tri-methylation of histone H3 at 'Lys-4' and transcriptional activation and thereby facilitates G1 to S phase transition (PubMed:23629655) . During myoblast differentiation, required to suppress inappropriate expression of S-phase-promoting genes and maintain expression of determination genes in quiescent cells (By similarity) .

Summary: Tissue Location: Widely expressed in both adult and fetal tissues (PubMed:12101424, PubMed:23958951) . Highest levels of expression observed in fetal thymus and kidney and in adult hematopoietic tissues, jejunum and cerebellum (PubMed:12101424, PubMed:23958951) . Isoform NKp44L: Not detected on circulating cells from healthy individuals, but is expressed on a large panel of tumor and transformed cells (PubMed:23958951) .

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB

Dilution: WB--1:1000

Synonyms: Histone-lysine N-methyltransferase 2E, Lysine N-methyltransferase 2E, Myeloid/lymphoid or mixed-lineage leukemia protein 5, KMT2E, MLL5