MMP-13 Inhibitor Screening Kit (Fluorometric) - K2067

K2067 | MMP-13 Inhibitor Screening Kit (Fluorometric) DataSheet

Matrix metalloproteinase-13 (MMP-13; Collagenase-3) is a zinc-dependent endopeptidase family member that is capable of degrading many protein components of the extracellular matrix (ECM) including type II collagen in articular cartilage and bone. It is produced and secreted as a 60-kDa inactive, precursor form (proMMP-13), which is activated to a 48-kDa form by extracellular proteinases such as plasmin, MMP-2, MMP-3 and MMP-14. MMP-13 has wide substrate specificity, as it degrades collagen types (I-IV) but preferentially hydrolyzes type II collagen. Additionally, it cleaves other ECM molecules including fibronectin, aggrecan, osteonectin etc. MMP-13 plays a major role in ECM remodeling during fetal bone development, post-natal bone remodeling, tissue remodeling etc. It is overexpressed in numerous pathological conditions including rheumatoid arthritis, osteoarthritis, atherosclerosis, aortic aneurysms, cancer etc. Thus, developing and screening of selective MMP-13 inhibitors is under intensive investigation for therapeutic intervention. ’s MMP-13 Inhibitor Screening Assay Kit provides a quick, easy and sensitive assay for screening, studying and characterizing potential inhibitors of MMP-13. In this assay, MMP-13 hydrolyzes a FRET-based MMP-13 substrate and releases unquenched by the catalysis of fluorescent group, Mca, which is detected fluorometrically at Ex/Em = 325/393 nm. A potent, specific MMP-13 inhibitor is also included in the kit.

Alternate Name : MMP-13 Inhibitor Screening Assay (Fluorometric),MMP-13 Inhibitor Screening Kit,MMP-13 Kit (Fluorometric),MMP-13 Inhibitor Screening (Fluorometric),MMP-13 Inhibitor Screening Assay Kit (Fluorometric),MMP-13 Inhibitor Screening Assay

Tag Line : For screening, studying and characterizing potential inhibitors of MMP-13

Summary : ’s MMP-13 Inhibitor Screening Assay Kit provides a quick, easy and sensitive assay for screening, studying and characterizing potential inhibitors of MMP-13. In this assay, MMP-13 hydrolyzes a FRET-based MMP-13 substrate and releases unquenched by the catalysis of fluorescent group, Mca, which is detected fluorometrically at Ex/Em = 325/393 nm. A potent, specific MMP-13 inhibitor is also included in the kit.

Detection Method : Fluorescence (Ex/Em = 325/393 nm)

Sample Type : Potential MMP-13 Inhibitors

Species Reactivity : Mammalian

Applications : Screening or characterizing MMP-13 inhibitors.

Features and Benefits : A potent, specific MMP-13 inhibitor is also included in the kit.,A quick, easy and sensitive assay for screening, studying and characterizing potential inhibitors of MMP-13.