MOG (Human) ELISA Kit - E4963

E4963 | MOG (Human) ELISA Kit DataSheet

Myelin oligodendrocyte glycoprotein (MOG) is a 26 kDa transmembrane glycoprotein that belongs to the immunoglobulin superfamily. MOG acts either as a cell surface receptor or a cell adhesion molecule. It is expressed exclusively by the oligodendrocytes in the central nervous system (CNS) and on the surface of myelin sheaths. It makes up about 0.05% of the myelin sheath and enables smooth electrical conduction of action potential. The extracellular IgV domain of MOG is accessible to the antibodies present in the extracellular milieu. As a result, the antibodies attack MOG and elicit a demyelinating autoantibody response. MOG antibodies are observed in disorders such as optic neuritis (ON), acute disseminated encephalomyelitis (ADEM), and transverse myelitis (TM). Hence, the detection of MOG antibodies has been implicated in the diagnosis of patients with CNS demyelination disorders. ’s MOG (Human) ELISA kit is based on the Sandwich principle to quantitatively measure the concentration of MOG in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and then washed with Wash Buffer. The HRP-Streptavidin is added and any unattached conjugates are washed off using Wash Buffer. The HRP enzymatic reaction is detected by the addition of TMB-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is directly proportional to the concentration of MOG in the sample or standard.

Alternate Name : BTN6, BTNL11, MOGIG2, NRCLP7, MOG

Tag Line : A Sandwich ELISA kit for quantitative measurement of Myelin-oligodendrocyte glycoprotein (MOG) in human serum, plasma, tissue lysates, and other biological fluids

Summary :

Detection Method : Absorbance (450 nm)

Sample Type : Tissue lysate Serum Plasma Other biological fluids

Species Reactivity : Human

Applications : Sandwich ELISA kit to quantitatively measure MOG in human serum, plasma, tissue lysate, and other biological fluids

Features and Benefits : Detection range: 0.156 – 10 ng/ml This Sandwich ELISA is highly sensitive and highly specific for the detection of MOG in human samples. There is no significant cross-reactivity or interference between MOG and analogues Sensitivity: 0.094 ng/ml Recovery range: 85 - 105% for normal human serum and plasma samples Assay Precision: Intra-Assay CV < 8% and Inter-Assay CV < 10%