Mouse 20S-PSM (20S-Proteasome) ELISA Kit | ELK6913

Mouse 20S-PSM (20S-Proteasome) ELISA Kit | ELK6913 | Gentaur UK, US & Europe Distribution

Alternative Names: CP; 20SPSM; 20S Core Particle

Species: Mouse

Assay Type: Competitive Inhibition

Sensitivity: 152.4 ng/mL

Standard: 30000 ng/mL

Detection Range: 468.75-30000 ng/mL

Sample Type: Serum, plasma and other biological fluids.

Assay Lenght: 2h

Research Area: Enzyme & Kinase

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 20S-Proteasome (20S-PSM) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 20S-Proteasome (20S-PSM) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 20S-Proteasome (20S-PSM) in the samples is then determined by comparing the OD of the samples to the standard curve.