Mouse 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial (CYP27B1) ELISA Kit | AE58946MO

Mouse 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial (CYP27B1) ELISA Kit | AE58946MO | Gentaur UK, US & Europe Distribution

Species Reactivity: Mouse (Mus musculus)

Abbreviation: CYP27B1

Alternative Name: CP2B; CYP1; CYP1alpha; CYP27B; P450c1; PDDR; VDD1; VDDR; VDDRI; VDR; 1alpha (OH) ase|25 hydroxyvitamin D3-1-alpha hydroxylase|25-Hydroxyvitamin D3 1alpha-hydroxylase|25-OHD-1 alpha-hydroxylase|25-hydr

Application: ELISA

Range: 0.625-40 ng/mL

Sensitivity: 0.235 ng/mL

Intra-Assay: ≤4.3%

Inter-Assay: ≤8.5%

Recovery: 0, 91

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate CYP27B1 in samples. An antibody specific for CYP27B1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCYP27B1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CYP27B1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CYP27B1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: CYP27B1 isa member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the inner mitochondrial membrane where it hydroxylates 25-hydroxyvitamin D3 at the 1alpha position. This reaction synthesizes 1alpha, 25-dihydroxyvitamin D3, the active form of vitamin D3, which binds to the vitamin D receptor and regulates calcium metabolism. Thus this enzyme regulates the level of biologically active vitamin D and plays an important role in calcium homeostasis. Mutations in this gene can result in vitamin D-dependent rickets type I.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .