Mouse 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase (CYP8B1) ELISA Kit | KTE71346

Mouse 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase (CYP8B1) ELISA Kit | KTE71346 | Gentaur UK, US & Europe Distribution

Application: This Mouse 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase (CYP8B1) ELISA Kit employs a two-site sandwich ELISA to quantitate CYP8B1 in samples. An antibody specific for CYP8B1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCYP8B1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CYP8B1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CYP8B1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase microplate
• Mouse 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase standard
• Mouse 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase (CYP8B1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse CYP8B1. No significant cross-reactivity or interference between Mouse CYP8B1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: CYP8B1 encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the conversion of 7 alpha-hydroxy-4-cholesten-3-one into 7-alpha, 12-alpha-dihydroxy-4-cholesten-3-one. The balance between these two steroids determines the relative amounts of cholic acid and chenodeoxycholic acid both of which are secreted in the bile and affect the solubility of cholesterol. This gene is unique among the cytochrome P450 genes in that it is intronless.

Alternative Names: CYP8B1; CP8B; CYP12; FLJ17826; 7 alpha-hydroxy-4-cholesten-3-one 12-alpha-hydroxylase; cytochrome P450; subfamily VIIIB (sterol 12-alpha-hydroxylase) ; polypeptide 1; sterol 12-alpha-hydroxylase

Search name: CYP8B1; CP8B; CYP12; FLJ17826; 7 alpha-hydroxy-4-cholesten-3-one 12-alpha-hydroxylase; cytochrome P450; subfamily VIIIB (sterol 12-alpha-hydroxylase) ; polypeptide 1; sterol 12-alpha-hydroxylase

Tag: CYP8B1