Mouse a1M (Alpha-1-Microglobulin/Bikunin Precursor) ELISA Kit | ELK5209

Mouse a1M (Alpha-1-Microglobulin/Bikunin Precursor) ELISA Kit | ELK5209 | Gentaur UK, US & Europe Distribution

Alternative Names: AMBP; UTI; HCP; EDC1; HI30; IATIL; ITILC; ITI; ITIL; Alpha 1 Microglobulin/Bikunin Precursor; Growth-inhibiting protein 19; Uristatin; Uronic-Acid-Rich Protein; Trypstatin

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.71 ng/mL

Standard: 100 ng/mL

Detection Range: 1.57-100 ng/mL

Sample Type: serum, plasma and other biological fluids

Assay Lenght: 3.5h

Research Area: Infection immunity;Immune molecule;Kidney biomarker

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-1-Microglobulin/Bikunin Precursor (a1M) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Alpha-1-Microglobulin/Bikunin Precursor (a1M) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-1-Microglobulin/Bikunin Precursor (a1M), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha-1-Microglobulin/Bikunin Precursor (a1M) in the samples is then determined by comparing the OD of the samples to the standard curve.