Mouse a1M (Alpha-1-Microglobulin/Bikunin Precursor) ELISA Kit | ELK5551

Mouse a1M (Alpha-1-Microglobulin/Bikunin Precursor) ELISA Kit | ELK5551 | Gentaur UK, US & Europe Distribution

Alternative Names: AMBP; UTI; HCP; EDC1; HI30; IATIL; ITILC; ITI; ITIL; Alpha 1 Microglobulin/Bikunin Precursor; Growth-inhibiting protein 19; Uristatin; Uronic-Acid-Rich Protein; Trypstatin

Species: Mouse

Assay Type: Competitive Inhibition

Sensitivity: 0.94 μg/mL

Standard: 200 μg/mL

Detection Range: 3.13-200 μg/mL

Sample Type: serum, plasma and other biological fluids

Assay Lenght: 2h

Research Area: Infection immunity;Immune molecule;Kidney biomarker

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Alpha-1-Microglobulin/Bikunin Precursor (a1M) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Alpha-1-Microglobulin/Bikunin Precursor (a1M) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha-1-Microglobulin/Bikunin Precursor (a1M) in the samples is then determined by comparing the OD of the samples to the standard curve.