Mouse ACAT2 (Acetyl Coenzyme A Acetyltransferase 2) ELISA Kit | ELK7071

Mouse ACAT2 (Acetyl Coenzyme A Acetyltransferase 2) ELISA Kit | ELK7071 | Gentaur UK, US & Europe Distribution

Alternative Names: ACTL; Acetoacetyl Coenzyme A Thiolase; Acetyl-CoA transferase-like protein; Cytosolic acetoacetyl-CoA thiolase

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.059 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Acetyl Coenzyme A Acetyltransferase 2 (ACAT2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Acetyl Coenzyme A Acetyltransferase 2 (ACAT2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Acetyl Coenzyme A Acetyltransferase 2 (ACAT2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Acetyl Coenzyme A Acetyltransferase 2 (ACAT2) in the samples is then determined by comparing the OD of the samples to the standard curve.