Mouse Acetyl-CoA acetyltransferase, mitochondrial (ACAT1) ELISA Kit | KTE70536

Mouse Acetyl-CoA acetyltransferase, mitochondrial (ACAT1) ELISA Kit | KTE70536 | Gentaur UK, US & Europe Distribution

Application: This Mouse Acetyl-CoA acetyltransferase, mitochondrial (ACAT1) ELISA Kit employs a two-site sandwich ELISA to quantitate ACAT1 in samples. An antibody specific for ACAT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACAT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACAT1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACAT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Acetyl-CoA acetyltransferase, mitochondrial microplate
• Mouse Acetyl-CoA acetyltransferase, mitochondrial standard
• Mouse Acetyl-CoA acetyltransferase, mitochondrial detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Acetyl-CoA acetyltransferase, mitochondrial (ACAT1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse ACAT1. No significant cross-reactivity or interference between Mouse ACAT1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: ACAT1 is a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone.The 427-amino acid precursor had a molecular mass of 45.2 kD. The sequence included a 33-residue leader peptide and a 394-amino acid subunit of the mature enzyme, which had a molecular mass of 41.4 kD. In all 4 cell lines, the T2 mRNA had the same 1.7-kb transcript as that of the control; however, content was reduced in 2 cell lines and normal in the other 2. Human T2 is a homotetramer of 41-kD subunits.

Alternative Names: ACAT1; ACAT; MAT; T2; THIL; acetoacetyl Coenzyme A thiolase; mitochondrial acetoacetyl-CoA thiolase

Search name: ACAT1; ACAT; MAT; T2; THIL; acetoacetyl Coenzyme A thiolase; mitochondrial acetoacetyl-CoA thiolase

Tag: ACAT1