Mouse ADCYAP1 (Adenylate Cyclase Activating Polypeptide 1, Pituitary) ELISA Kit | ELK2658

Mouse ADCYAP1 (Adenylate Cyclase Activating Polypeptide 1, Pituitary) ELISA Kit | ELK2658 | Gentaur UK, US & Europe Distribution

Alternative Names: PACAP; PRP-48; Pituitary Adenylate Cyclase Activating Peptide

Species: Mouse

Assay Type: Sandwich

Sensitivity: 3.2 pg/mL

Standard: 500 pg/mL

Detection Range: 7.82-500 pg/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids

Assay Lenght: 3.5h

Research Area: Endocrinology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) in the samples is then determined by comparing the OD of the samples to the standard curve.