Mouse ALOX5 (Arachidonate-5-Lipoxygenase) ELISA Kit | ELK3188

Mouse ALOX5 (Arachidonate-5-Lipoxygenase) ELISA Kit | ELK3188 | Gentaur UK, US & Europe Distribution

Alternative Names: LOX5; 5-LOX; ALOX-5; 5-LO; 5LPG; LOG5; Lipoxygenase 5

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.065 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase;Metabolic pathway;Tumor immunity;Cardiovascular biology;Neuro science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arachidonate-5-Lipoxygenase (ALOX5) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Arachidonate-5-Lipoxygenase (ALOX5) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Arachidonate-5-Lipoxygenase (ALOX5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Arachidonate-5-Lipoxygenase (ALOX5) in the samples is then determined by comparing the OD of the samples to the standard curve.