Mouse Angiopoietin-related protein 4 (ANGPTL4) ELISA Kit | KTE71376

Mouse Angiopoietin-related protein 4 (ANGPTL4) ELISA Kit | KTE71376 | Gentaur UK, US & Europe Distribution

Application: This Mouse Angiopoietin-related protein 4 (ANGPTL4) ELISA Kit employs a two-site sandwich ELISA to quantitate ANGPTL4 in samples. An antibody specific for ANGPTL4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyANGPTL4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ANGPTL4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ANGPTL4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Angiopoietin-related protein 4 microplate
• Mouse Angiopoietin-related protein 4 standard
• Mouse Angiopoietin-related protein 4 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Angiopoietin-related protein 4 (ANGPTL4) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse ANGPTL4. No significant cross-reactivity or interference between Mouse ANGPTL4 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: ANGPTL4 (Angiopoietin Like 4) is a Protein Coding gene. Diseases associated with ANGPTL4 include Plasma Triglyceride Level Qtl, Low and Severe Nonproliferative Diabetic Retinopathy. Among its related pathways are Regulation of lipid metabolism by Peroxisome proliferator-activated receptor alpha (PPARalpha) and Metabolism. GO annotations related to this gene include enzyme inhibitor activity. An important paralog of this gene is ANGPTL3. Protein with hypoxia-induced expression in endothelial cells. May act as a regulator of angiogenesis and modulate tumorigenesis. Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. May exert a protective function on endothelial cells through an endocrine action. It is directly involved in regulating glucose homeostasis, lipid metabolism, and insulin sensitivity. In response to hypoxia, the unprocessed form of the protein accumulates in the subendothelial extracellular matrix (ECM) . The matrix-associated and immobilized unprocessed form limits the formation of actin stress fibers and focal contacts in the adhering endothelial cells and inhibits their adhesion. It also decreases motility of endothelial cells and inhibits the sprouting and tube formation.

Alternative Names: ANGPTL4; ANGPTL2; ARP4; FIAF; HFARP; NL2; PGAR; pp1158; PPARG angiopoietin related protein; angiopoietin-like 4 protein; fasting-induced adipose factor; hepatic angiopoietin-related protein; hepatic fibrinogen/a

Search name: ANGPTL4; ANGPTL2; ARP4; FIAF; HFARP; NL2; PGAR; pp1158; PPARG angiopoietin related protein; angiopoietin-like 4 protein; fasting-induced adipose factor; hepatic angiopoietin-related protein; hepatic fibrinogen/a

Tag: ANGPTL4