Mouse Anti-Human PDGFR-alpha Antibody | 101-M42

Mouse Anti-Human PDGFR-alpha Antibody | 101-M42 | Gentaur UK, US & Europe Distribution

Species: Anti-Human

Host / biotech: Mouse

Comment: N/A

Label: N/A

Clone / Antibody feature: (#8A89)

Subcategory: Monoclonal Antibody

Category: Antibody

Synonyms: PDGFRA; CD140A; PDGFR2; PDGFR-2; RHEPDGFRA

Isotype: IgG1

Application: WB, IHC (P)

Detection Range: WB: 1:400-1000; IHC: 1:50-500

Species Reactivity/Cross reactivity: Human

Antigen: N-terminal recombinant human PDGFR alpha fragment

Description: PDGF is a major serum mitogen that can exist as a homo- or heterodimeric protein consisting of disulfidelinked PDGF-A and PDGF-B chains. The PDGF-AA, PDGF-BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Whereas PDGF Rα binds all three PDGF isoforms with high affinity, PDGF Rβ binds PDG-FBB and AB, but not PDGFAA. Both PDGF Rα and PDGF Rβ are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for MCSF, SCF and Flt3 ligand. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. PDGF binding induces receptor homo- and heterodimerization and signal transduction. The expression of the α and β receptors is independently regulated in various cell types. Only PDGF Rα is expressed in oligodendrocyte progenitor cells, mesothelial cell and liver endothelial cells. Soluble PDGFRα has been detected in cell conditioned medium and human plasma. Recombinant soluble PDGF Rα binds PDGF with high affinity and is a potent PDGF antagonist.

Purity Confirmation: N/A

Endotoxin: N/A

Formulation: lyophilized

Storage Handling Stability: Lyophilized samples are stable for 2 years from date of receipt when stored at -20°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity.

Reconstituation: Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.

Molecular Weight: N/A

Lenght (aa): N/A

Protein Sequence: N/A

NCBI Gene ID: 5156