Mouse ATF6 (Activating Transcription Factor 6) ELISA Kit | ELK4479

Mouse ATF6 (Activating Transcription Factor 6) ELISA Kit | ELK4479 | Gentaur UK, US & Europe Distribution

Alternative Names: Processed cyclic AMP-dependent transcription factor ATF-6 alpha

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.126 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Signal transduction;Developmental science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Activating Transcription Factor 6 (ATF6) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Activating Transcription Factor 6 (ATF6) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Activating Transcription Factor 6 (ATF6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Activating Transcription Factor 6 (ATF6) in the samples is then determined by comparing the OD of the samples to the standard curve.