Mouse ATP2B2 (ATPase, Ca++ Transporting, Plasma Membrane 2) ELISA Kit | ELK6053

Mouse ATP2B2 (ATPase, Ca++ Transporting, Plasma Membrane 2) ELISA Kit | ELK6053 | Gentaur UK, US & Europe Distribution

Alternative Names: PMCA2; Plasma Membrane Ca2+ Pump 2; Plasma Membrane Calcium-Transporting ATPase 2

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.058 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATPase, Ca++ Transporting, Plasma Membrane 2 (ATP2B2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to ATPase, Ca++ Transporting, Plasma Membrane 2 (ATP2B2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATPase, Ca++ Transporting, Plasma Membrane 2 (ATP2B2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ATPase, Ca++ Transporting, Plasma Membrane 2 (ATP2B2) in the samples is then determined by comparing the OD of the samples to the standard curve.