Mouse ATP7b (ATPase, Cu++ Transporting Beta Polypeptide) ELISA Kit | ELK6926

Mouse ATP7b (ATPase, Cu++ Transporting Beta Polypeptide) ELISA Kit | ELK6926 | Gentaur UK, US & Europe Distribution

Alternative Names: PWD; WC1; WD; WND; Wilson Disease Protein; Copper pump 2; Wilson disease-associated protein

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.055 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATPase, Cu++ Transporting Beta Polypeptide (ATP7b) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to ATPase, Cu++ Transporting Beta Polypeptide (ATP7b) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATPase, Cu++ Transporting Beta Polypeptide (ATP7b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ATPase, Cu++ Transporting Beta Polypeptide (ATP7b) in the samples is then determined by comparing the OD of the samples to the standard curve.