Mouse AZU1 (Azurocidin 1) ELISA Kit | ELK8378

Mouse AZU1 (Azurocidin 1) ELISA Kit | ELK8378 | Gentaur UK, US & Europe Distribution

Alternative Names: AZAMP; AZU; HBP; HUMAZUR; NAZC; CAP37; Cationic Antimicrobial Protein 37; Heparin-Binding Protein; Neutrophil Azurocidin

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.09ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase;Infection immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Azurocidin 1 (AZU1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Azurocidin 1 (AZU1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Azurocidin 1 (AZU1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Azurocidin 1 (AZU1) in the samples is then determined by comparing the OD of the samples to the standard curve.