Mouse BCKDHB (2-oxoisovalerate dehydrogenase subunit beta, mitochondrial) ELISA Kit | ELK9525

Mouse BCKDHB (2-oxoisovalerate dehydrogenase subunit beta, mitochondrial) ELISA Kit | ELK9525 | Gentaur UK, US & Europe Distribution

Alternative Names: 2-oxoisovalerate dehydrogenase subunit beta, mitochondrial; Branched-chain alpha-keto acid dehydrogenase E1 component beta chain; BCKDE1B; BCKDH E1-beta; Bckdhb

Species: Mouse

Assay Type: Sandwich

Sensitivity: 12.2 pg/mL

Standard: 2000 pg/mL

Detection Range: 31.25-2000 pg/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 2-oxoisovalerate dehydrogenase subunit beta, mitochondrial (BCKDHB) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 2-oxoisovalerate dehydrogenase subunit beta, mitochondrial (BCKDHB) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 2-oxoisovalerate dehydrogenase subunit beta, mitochondrial (BCKDHB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 2-oxoisovalerate dehydrogenase subunit beta, mitochondrial (BCKDHB) in the samples is then determined by comparing the OD of the samples to the standard curve.