Mouse Bcl2L (B-Cell CLL/Lymphoma 2 Like Protein) ELISA Kit | ELK7209

Mouse Bcl2L (B-Cell CLL/Lymphoma 2 Like Protein) ELISA Kit | ELK7209 | Gentaur UK, US & Europe Distribution

Alternative Names: BCL-XL/S; BCL2L1; BCLX; Bcl-X; bcl-xL; bcl-xS; Apoptosis regulator Bcl-X; Bcl 2 like 1 protein

Species: Mouse

Assay Type: Sandwich

Sensitivity: 3.1 pg/mL

Standard: 500 pg/mL

Detection Range: 7.82-500 pg/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Immune molecule

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to B-Cell CLL/Lymphoma 2 Like Protein (Bcl2L) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to B-Cell CLL/Lymphoma 2 Like Protein (Bcl2L) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain B-Cell CLL/Lymphoma 2 Like Protein (Bcl2L), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of B-Cell CLL/Lymphoma 2 Like Protein (Bcl2L) in the samples is then determined by comparing the OD of the samples to the standard curve.