Mouse BMP-binding endothelial regulator protein (BMPER) ELISA Kit | KTE71407

Mouse BMP-binding endothelial regulator protein (BMPER) ELISA Kit | KTE71407 | Gentaur UK, US & Europe Distribution

Application: This Mouse BMP-binding endothelial regulator protein (BMPER) ELISA Kit employs a two-site sandwich ELISA to quantitate BMPER in samples. An antibody specific for BMPER has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyBMPER present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BMPER is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BMPER bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse BMP-binding endothelial regulator protein microplate
• Mouse BMP-binding endothelial regulator protein standard
• Mouse BMP-binding endothelial regulator protein detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse BMP-binding endothelial regulator protein (BMPER) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse BMPER. No significant cross-reactivity or interference between Mouse BMPER and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: BMPER contains a 39-amino acid signal peptide at the N terminus, 5 tandem cysteine-rich von Willebrand C (VWC) -like domains, 1 von Willebrand D domain, and a C-terminal trypsin inhibitor domain. Expression of recombinant Bmper showed the presence of full-length protein and a 42-kD C-terminal proteolytic fragment. In adult mice, expression of Bmper was high in heart, lung, and skin and low in brain. In situ hybridization of mouse tissue showed dynamic expression of Bmper during embryonic development. At embryonic day 9.5, Bmper staining was seen in the lamina reuniens adjacent to the telencephalic vesicle and in migrating branchial arch cells. At embryonic day 10.5, staining was seen in endothelial cells around the dorsal aorta and in adjacent migrating or mesenchymal cells.

Alternative Names: BMPER; CRIM3; CV-2; CV2; BMP-binding endothelial regulator precursor protein; OTTHUMP00000158859; bone morphogenetic protein-binding endothelial cell precursor-derived regulator; crossveinless 2; crossveinless

Search name: BMPER; CRIM3; CV-2; CV2; BMP-binding endothelial regulator precursor protein; OTTHUMP00000158859; bone morphogenetic protein-binding endothelial cell precursor-derived regulator; crossveinless 2; crossveinless

Tag: BMPER