Mouse BMX (BMX Non Receptor Tyrosine Kinase) ELISA Kit | ELK8182

Mouse BMX (BMX Non Receptor Tyrosine Kinase) ELISA Kit | ELK8182 | Gentaur UK, US & Europe Distribution

Alternative Names: ETK; PSCTK3; NTK38; Epithelial and endothelial tyrosine kinase; Bone marrow tyrosine kinase gene in chromosome X; Cytoplasmic tyrosine-protein kinase BMX

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.113 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to BMX Non Receptor Tyrosine Kinase (BMX) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to BMX Non Receptor Tyrosine Kinase (BMX) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain BMX Non Receptor Tyrosine Kinase (BMX), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of BMX Non Receptor Tyrosine Kinase (BMX) in the samples is then determined by comparing the OD of the samples to the standard curve.