Mouse Bone morphogenetic protein 6 (BMP-6) ELISA Kit | KTE71554

Mouse Bone morphogenetic protein 6 (BMP-6) ELISA Kit | KTE71554 | Gentaur UK, US & Europe Distribution

Application: This Mouse Bone morphogenetic protein 6 (BMP-6) ELISA Kit employs a two-site sandwich ELISA to quantitate BMP6 in samples. An antibody specific for BMP6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyBMP6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BMP6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BMP6 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Bone morphogenetic protein 6 microplate
• Mouse Bone morphogenetic protein 6 standard
• Mouse Bone morphogenetic protein 6 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Bone morphogenetic protein 6 (BMP-6) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse BMP6. No significant cross-reactivity or interference between Mouse BMP6 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Bone morphogenetic proteins, such as BMP6, belong to the transforming growth factor-beta superfamily of regulatory molecules (Rickard et al., 1998) .Rickard et al. (1998) presented evidence that the skeletal effects of estrogen on bone and cartilage may be mediated by increased production of BMP6 by osteoblasts. They investigated the effect of estrogen on BMP production in 2 estrogen-responsive, human immortalized cell lines that display the mature osteoblast phenotype.Tamada et al. (1998) found that the promoter region of the BMP6 gene lacks a canonical TATA box, but has a GC-rich region with 2 SP1-binding sites, an inverted CCAAT element, and a putative tramtrack responsive element. Reporter gene assays suggested that the BMP6 promoter has osteogenic cell specificity.

Alternative Names: BMP6; VGR; VGR1; Vg1-related sequence; vegetal related growth factor (TGFB-related) ; vegetal-related (TGFB related) cytokine

Search name: BMP6; VGR; VGR1; Vg1-related sequence; vegetal related growth factor (TGFB-related) ; vegetal-related (TGFB related) cytokine

Tag: BMP6