Mouse C3 (Complement Component 3) ELISA Kit | ELK1640

Mouse C3 (Complement Component 3) ELISA Kit | ELK1640 | Gentaur UK, US & Europe Distribution

Alternative Names: ASP; CPAMD1; Complement Protein C3; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1; C3a anaphylatoxin; Acylation stimulating protein

Species: Mouse

Assay Type: Sandwich

Sensitivity: 1.39 ng/mL

Standard: 200 ng/mL

Detection Range: 3.13-200 ng/mL

Sample Type: serum, plasma and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction;Infection immunity;Immune molecule

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Component 3 (C3) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Complement Component 3 (C3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Component 3 (C3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Complement Component 3 (C3) in the samples is then determined by comparing the OD of the samples to the standard curve.