Mouse C5 (Complement Component 5) ELISA Kit | ELK2944

Mouse C5 (Complement Component 5) ELISA Kit | ELK2944 | Gentaur UK, US & Europe Distribution

Alternative Names: CPAMD4; C5a anaphylatoxin; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.073 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: serum, plasma and other biological fluids

Assay Lenght: 3.5h

Research Area: Metabolic pathway;Infection immunity;Immune molecule;Hematology;Autoimmunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Component 5 (C5) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Complement Component 5 (C5) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Component 5 (C5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Complement Component 5 (C5) in the samples is then determined by comparing the OD of the samples to the standard curve.