Mouse CA125 (Carbohydrate Antigen 125) ELISA Kit | ELK1479

Mouse CA125 (Carbohydrate Antigen 125) ELISA Kit | ELK1479 | Gentaur UK, US & Europe Distribution

Alternative Names: MUC16; Cancer Antigen 125; Mucin 16, Cell Surface Associated; Ovarian Cancer-Related Tumor Marker CA125; Ovarian Carcinoma Antigen CA125

Species: Mouse

Assay Type: Sandwich

Sensitivity: 6.5 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: serum, plasma, tissue homogenates and other biological fluids

Assay Lenght: 3.5h

Research Area: Tumor immunity;Reproductive science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carbohydrate Antigen 125 (CA125) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Carbohydrate Antigen 125 (CA125) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carbohydrate Antigen 125 (CA125), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Carbohydrate Antigen 125 (CA125) in the samples is then determined by comparing the OD of the samples to the standard curve.