Mouse CACNa1E (Calcium Channel, Voltage Dependent, R-Type, Alpha 1E Subunit) ELISA Kit | ELK6875

Mouse CACNa1E (Calcium Channel, Voltage Dependent, R-Type, Alpha 1E Subunit) ELISA Kit | ELK6875 | Gentaur UK, US & Europe Distribution

Alternative Names: BII; CACNL1A6; CACH6; Brain calcium channel II; Calcium channel, L type, alpha-1 polypeptide, isoform 6; Voltage-gated calcium channel subunit alpha Cav2.3

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.125 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calcium Channel, Voltage Dependent, R-Type, Alpha 1E Subunit (CACNa1E) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Calcium Channel, Voltage Dependent, R-Type, Alpha 1E Subunit (CACNa1E) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calcium Channel, Voltage Dependent, R-Type, Alpha 1E Subunit (CACNa1E), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calcium Channel, Voltage Dependent, R-Type, Alpha 1E Subunit (CACNa1E) in the samples is then determined by comparing the OD of the samples to the standard curve.