Mouse Calcium-independent phospholipase A2 (PLA2G6) ELISA Kit | KTE70738

Mouse Calcium-independent phospholipase A2 (PLA2G6) ELISA Kit | KTE70738 | Gentaur UK, US & Europe Distribution

Application: This Mouse Calcium-independent phospholipase A2 (PLA2G6) ELISA Kit employs a two-site sandwich ELISA to quantitate PLA2G6 in samples. An antibody specific for PLA2G6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLA2G6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLA2G6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLA2G6 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Calcium-independent phospholipase A2 microplate
• Mouse Calcium-independent phospholipase A2 standard
• Mouse Calcium-independent phospholipase A2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Calcium-independent phospholipase A2 (PLA2G6) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PLA2G6. No significant cross-reactivity or interference between Mouse PLA2G6 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: 85 kDa calcium-independent phospholipase A2 is is an A2 phospholipase, a class of enzyme that catalyzes the release of fatty acids from phospholipids. The encoded protein may play a role in phospholipid remodelling, arachidonic acid release, leukotriene and prostaglandin synthesis, fas-mediated apoptosis, and transmembrane ion flux in glucose-stimulated B-cells. Several transcript variants encoding multiple isoforms have been described, but the full-length nature of only two of them have been determined to date.Tang et al. (1997) purified an 85-kD cytosolic calcium-independent PLA2, termed iPLA2, from Chinese hamster ovary (CHO) cells. They isolated a partial human cDNA from a Burkitt lymphoma cDNA library by screening with a CHO cDNA. Northern blot analysis revealed that iPLA2 is expressed in a variety of rodent tissues.

Alternative Names: PLA2G6; CTA-228A9.2; CaI-PLA2; GVI; INAD1; IPLA2-VIA; PARK14; PLA2; PNPLA9; iPLA2; OTTHUMP00000199457; cytosolic; calcium-independent phospholipase A2; patatin-like phosph; CaI-PLA2; GVI; INAD1; Ca2+ Independent PLA2; 85 kDa Calcium-Independent Phospholipase A2; Phospholipase A2, group VI (Cytosolic, Calcium-Independent)

Search name: PLA2G6; CTA-228A9.2; CaI-PLA2; GVI; INAD1; IPLA2-VIA; PARK14; PLA2; PNPLA9; iPLA2; OTTHUMP00000199457; cytosolic; calcium-independent phospholipase A2; patatin-like phosph; CaI-PLA2; GVI; INAD1; Ca2+ Independent PLA2; 85 kDa Calcium-Independent Phospholipase A2; Phospholipase A2, group VI (Cytosolic, Calcium-Independent)

Tag: PLA2G6