Mouse CB10

Mouse CB10 - Cat Number: 9061 From .

Research Field: Arthritis

Clonality: N/A

Cross-Reactivity:

Host Origin: N/A

Applications: N/A

Isotype: N/A

Detection Range: N/A

Sample Type: N/A

Concentration: N/A

Immunogen:

DESCRIPTION: Cyanogen bromide cleaved peptide 10 (CB10) of mouse type II collagen

APPLICATION: Use as an antigen to stimulate T-cells for T-cell proliferation assays and as an ELISA antigen
for assaying antibodies to type II collagen.
NOTE: For assaying antibodies that react to native type II collagen using this peptide fragment,
renature this peptide by stepwise cooling according to the method described by Igarashi et al
(1) and Terato et al (2).
QUANTITY: 0.5 mg
FORM: Lyophilized powder
SOURCE: Mouse Type II Collagen
MOLECULAR WEIGHT: Approximately 39 kDa (a peptide representing residues 551 - 1897 of type II collagen) (3)
PURITY: >95% by 10% SDS-PAGE
STORAGE: 4°C in the dark for lyophilized form and -20°C for solution form. This product is stable for 2
years as supplied. Peptide fragments will gradually degrade under neutral conditions.
STABILITY: 2 years
NOTES: Soluble in 0.05M acetic acid, neutral buffers containing 0.15M NaCl, and cell culture medium
REFERENCES:
1. S. Igarashi, A. Kang, J. Gross, Biochem. Biophyis. Res. Comm. 38: 697-701 (1970)
2. K. Terato, M. Cremer, K. Hasty, et al. Collagen Relat. Res. 5: 469-480 (1985)
3. E. Miller and L. Lunde, Biochemistry 12: 3153-3159 (1973)