Mouse CEBPb (CCAAT/Enhancer Binding Protein Beta) ELISA Kit | ELK4320

Mouse CEBPb (CCAAT/Enhancer Binding Protein Beta) ELISA Kit | ELK4320 | Gentaur UK, US & Europe Distribution

Alternative Names: CRP2; IL6DBP; LAP; NF-IL6; TCF5; C/EBP-Beta; Liver-Enriched Transcriptional Activator Protein; Nuclear Factor Of Interleukin 6; Interleukin 6-Dependent DNA-Binding Protein

Species: Mouse

Assay Type: Sandwich

Sensitivity: 33 pg/mL

Standard: 5000 pg/mL

Detection Range: 78.13-5000 pg/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CCAAT/Enhancer Binding Protein Beta (CEBPb) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to CCAAT/Enhancer Binding Protein Beta (CEBPb) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CCAAT/Enhancer Binding Protein Beta (CEBPb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CCAAT/Enhancer Binding Protein Beta (CEBPb) in the samples is then determined by comparing the OD of the samples to the standard curve.