Mouse CHAF1B (Chromatin Assembly Factor 1, Subunit B) ELISA Kit | ELK6320

Mouse CHAF1B (Chromatin Assembly Factor 1, Subunit B) ELISA Kit | ELK6320 | Gentaur UK, US & Europe Distribution

Alternative Names: CAF-1; CAF-IP60; CAF1; CAF1A; CAF1P60; MPHOSPH7; MPP7; M-Phase Phosphoprotein 7; Chromatin Assembly Factor I, p60 subunit; M-phase phosphoprotein 7

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.052 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates, cell Lysates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chromatin Assembly Factor 1, Subunit B (CHAF1B) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chromatin Assembly Factor 1, Subunit B (CHAF1B) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chromatin Assembly Factor 1, Subunit B (CHAF1B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chromatin Assembly Factor 1, Subunit B (CHAF1B) in the samples is then determined by comparing the OD of the samples to the standard curve.