Mouse Chymotrypsin-like elastase family member 2A (CEL) ELISA Kit | AE59114MO

Mouse Chymotrypsin-like elastase family member 2A (CEL) ELISA Kit | AE59114MO | Gentaur UK, US & Europe Distribution

Species Reactivity: Mouse (Mus musculus)

Abbreviation: CEL

Alternative Name: BAL; BSDL; BSSL; CELL; CEase; FAP; FAPP; LIPA; MODY8; bile salt-dependent lipase; oncofetal isoform|bile salt-stimulated carboxyl ester lipase|bile salt-stimulated lipase|bile-salt-activated lipase|

Application: ELISA

Range: 31.25-2000 pg/mL

Sensitivity: 14.6 pg/mL

Intra-Assay: ≤5.6%

Inter-Assay: ≤9.2%

Recovery: 0, 98

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate CEL in samples. An antibody specific for CEL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCEL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CEL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CEL bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Like most of the human elastases, elastase 2A is secreted from the pancreas as a zymogen. In other species, elastase 2A has been shown to preferentially cleave proteins after leucine, methionine, and phenylalanine residues. Clinical literature that describes human elastase 1 activity in the pancreas is actually referring to elastase 2A. The deduced 269-amino acid protein shares 55 to 84% identity with other mammalian elastases and 39% identity with bovine chymotrypsin A. Most homology resides in the N-terminal sequence and in the C-terminal half of the protein.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .