Mouse CLU (Clusterin) ELISA Kit | ELK1310

Mouse CLU (Clusterin) ELISA Kit | ELK1310 | Gentaur UK, US & Europe Distribution

Alternative Names: CLU; TRPM2; KUB1; CLI; APOJ; SGP2; AAG4; APO-J; SP-40; Apolipoprotein J; Complement Lysis Inhibitor; 40, Sulfated Glycoprotein 2; Testosterone-Repressed Prostate Message 2

Species: Mouse

Assay Type: Sandwich

Sensitivity: 7.3 ng/mL

Standard: 1200 ng/mL

Detection Range: 18.75-1200 ng/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Apoptosis;Tumor immunity;Infection immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Clusterin (CLU) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Clusterin (CLU) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Clusterin (CLU), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Clusterin (CLU) in the samples is then determined by comparing the OD of the samples to the standard curve.