Mouse Coagulation Factor XIII B Polypeptide (F13B) ELISA Kit | KTE71495

Mouse Coagulation Factor XIII B Polypeptide (F13B) ELISA Kit | KTE71495 | Gentaur UK, US & Europe Distribution

Application: This Mouse Coagulation Factor XIII B Polypeptide (F13B) ELISA Kit employs a two-site sandwich ELISA to quantitate F13B in samples. An antibody specific for F13B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyF13B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for F13B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of F13B bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Coagulation Factor XIII B Polypeptide microplate
• Mouse Coagulation Factor XIII B Polypeptide standard
• Mouse Coagulation Factor XIII B Polypeptide detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Coagulation Factor XIII B Polypeptide (F13B) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse F13B. No significant cross-reactivity or interference between Mouse F13B and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Coagulation factor XIII is the last zymogen to become activated in the blood coagulation cascade. Plasma factor XIII is a heterotetramer composed of 2 A subunits and 2 B subunits. The A subunits have catalytic function, and the B subunits do not have enzymatic activity and may serve as a plasma carrier molecules. Platelet factor XIII is comprised only of 2 A subunits, which are identical to those of plasma origin. Upon activation by the cleavage of the activation peptide by thrombin and in the presence of calcium ion, the plasma factor XIII dissociates its B subunits and yields the same active enzyme, factor XIIIa, as platelet factor XIII. This enzyme acts as a transglutaminase to catalyze the formation of gamma-glutamyl-epsilon-lysine crosslinking between fibrin molecules, thus stabilizing the fibrin clot.

Alternative Names: F13B; FXIIIB; TGase; coagulation factor XIII B subunit

Search name: F13B; FXIIIB; TGase; coagulation factor XIII B subunit

Tag: F13B