Mouse COL3 (Collagen Type III) ELISA Kit | ELK2572

Mouse COL3 (Collagen Type III) ELISA Kit | ELK2572 | Gentaur UK, US & Europe Distribution

Alternative Names: Collagen Type 3; Col 3; Collagen Type III; Col III

Species: Mouse

Assay Type: Sandwich

Sensitivity: 18.24 pg/mL

Standard: 3000 pg/mL

Detection Range: 46.88-3000 pg/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids

Assay Lenght: 3.5h

Research Area: Metabolic pathway;Cardiovascular biology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen Type III (COL3) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Collagen Type III (COL3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Collagen Type III (COL3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Collagen Type III (COL3) in the samples is then determined by comparing the OD of the samples to the standard curve.