Mouse COL5a2 (Collagen Type V Alpha 2) ELISA Kit | ELK6551

Mouse COL5a2 (Collagen Type V Alpha 2) ELISA Kit | ELK6551 | Gentaur UK, US & Europe Distribution

Alternative Names: COL5-A2; Collagen Alpha-2 (V) chain; AB Collagen

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.116 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Serum, plasma and other biological fluids.

Assay Lenght: 3.5h

Research Area: Metabolic pathway;Developmental science;Nutrition metabolism;Bone metabolism

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen Type V Alpha 2 (COL5a2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Collagen Type V Alpha 2 (COL5a2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Collagen Type V Alpha 2 (COL5a2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Collagen Type V Alpha 2 (COL5a2) in the samples is then determined by comparing the OD of the samples to the standard curve.