Mouse CRT (Calreticulin) ELISA Kit | ELK3182

Mouse CRT (Calreticulin) ELISA Kit | ELK3182 | Gentaur UK, US & Europe Distribution

Alternative Names: CALR; RO; SSA; CC1qR; ERp60; HACBP; grp60; CRTC; CRP55; Calregulin; Sicca Syndrome Antigen A; Autoantigen Ro; Endoplasmic reticulum resident protein 60

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.067 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: serum, plasma, tissue homogenates and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction;Metabolic pathway;Bone metabolism

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calreticulin (CRT) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Calreticulin (CRT) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calreticulin (CRT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calreticulin (CRT) in the samples is then determined by comparing the OD of the samples to the standard curve.