Mouse CTSA (Cathepsin A) ELISA Kit | ELK4299

Mouse CTSA (Cathepsin A) ELISA Kit | ELK4299 | Gentaur UK, US & Europe Distribution

Alternative Names: CTS-A; GLB2; GSL; NGBE; PPCA; PPGB; Protective Protein For Beta-Galactosidase; Galactosialidosis; Carboxypeptidase C; Carboxypeptidase L

Species: Mouse

Assay Type: Sandwich

Sensitivity: 2.74 pg/mL

Standard: 400 pg/mL

Detection Range: 6.25-400 pg/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cathepsin A (CTSA) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cathepsin A (CTSA) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cathepsin A (CTSA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cathepsin A (CTSA) in the samples is then determined by comparing the OD of the samples to the standard curve.