Mouse CX43 (Connexin 43) ELISA Kit | ELK2880

Mouse CX43 (Connexin 43) ELISA Kit | ELK2880 | Gentaur UK, US & Europe Distribution

Alternative Names: ODDD; GJAL; GJA1; GJ-A1; Gap junction 43 kDa heart protein; Gap Junction Protein Alpha 1; Oculodentodigital Dysplasia; Syndactyly Type III

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.065 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Cardiovascular biology;Neuro science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Connexin 43 (CX43) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Connexin 43 (CX43) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Connexin 43 (CX43), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Connexin 43 (CX43) in the samples is then determined by comparing the OD of the samples to the standard curve.