Mouse Cyclic AMP-dependent transcription factor ATF-2 (ATF2) ELISA Kit | AE61727MO

Mouse Cyclic AMP-dependent transcription factor ATF-2 (ATF2) ELISA Kit | AE61727MO | Gentaur UK, US & Europe Distribution

Species Reactivity: Mouse (Mus musculus)

Abbreviation: ATF2

Alternative Name: CRE-BP1; CREB2; HB16; MGC111558; TREB7; activating transcription factor 2 splice variant ATF2-var2|cAMP responsive element binding protein 2; formerly

Application: ELISA

Range: 31.25-2000 pg/mL

Sensitivity: 12.1 pg/mL

Intra-Assay: ≤6.9%

Inter-Assay: ≤11.3%

Recovery: 1, 01

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate ATF2 in samples. An antibody specific for ATF2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyATF2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ATF2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ATF2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Glial Cell Line-derived Neurotrophic Factor (GDNF) is a highly conserved neurotrophic factor. The recombinant form of this protein was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy.The protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. In addition to the transcript encoding GDNF, two additional alternative transcripts encoding distinct proteins, referred to as astrocyte-derived trophic factors, have also been described. Mutations in this gene may be associated with Hirschsprung disease.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .