Mouse Cytochrome b-245 heavy chain (CYBB) ELISA Kit | KTE71359

Mouse Cytochrome b-245 heavy chain (CYBB) ELISA Kit | KTE71359 | Gentaur UK, US & Europe Distribution

Application: This Mouse Cytochrome b-245 heavy chain (CYBB) ELISA Kit employs a two-site sandwich ELISA to quantitate CYBB in samples. An antibody specific for CYBB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCYBB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CYBB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CYBB bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Cytochrome b-245 heavy chain microplate
• Mouse Cytochrome b-245 heavy chain standard
• Mouse Cytochrome b-245 heavy chain detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Cytochrome b-245 heavy chain (CYBB) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse CYBB. No significant cross-reactivity or interference between Mouse CYBB and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Cytochrome b-245 heavy chain (CYBB) encodes the heavy chain component of a heterodimeric transmembrane ion transporter composed of both a heavy and a light chain. This transporter mediates the transfer of electrons from nicotinamide adenine dinucleotide phosphate (NADPH) to oxygen to generate superoxide. This reaction is important in the innate immune response to pathogens. However, increased activity of the encoded protein also leads to the generation of reactive oxygen species that result in oxidative stress and can cause tissue damage. Conversely, loss of function of the related gene in human causes chronic granulomatous disease. Alternative splicing results in multiple transcript variants, although the full-length nature of some of these variants has not been determined.

Alternative Names: CYBB; CGD; GP91-1; GP91-PHOX; GP91PHOX; NOX2; p91-PHOX; NADPH oxidase 2; cytochrome b-245 beta polypeptide

Search name: CYBB; CGD; GP91-1; GP91-PHOX; GP91PHOX; NOX2; p91-PHOX; NADPH oxidase 2; cytochrome b-245 beta polypeptide

Tag: CYBB