Mouse DBP (Vitamin D Binding Protein) ELISA Kit | ELK4285

Mouse DBP (Vitamin D Binding Protein) ELISA Kit | ELK4285 | Gentaur UK, US & Europe Distribution

Alternative Names: GC; VDBG; VDBP; Group-Specific Component; Gc-globulin

Species: Mouse

Assay Type: Sandwich

Sensitivity: 2.76 ng/mL

Standard: 400 ng/mL

Detection Range: 6.25-400 ng/mL

Sample Type: Serum, plasma and other biological fluids.

Assay Lenght: 3.5h

Research Area: Metabolic pathway;Tumor immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vitamin D Binding Protein (DBP) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Vitamin D Binding Protein (DBP) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vitamin D Binding Protein (DBP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Vitamin D Binding Protein (DBP) in the samples is then determined by comparing the OD of the samples to the standard curve.