Mouse Deoxyribonuclease-1 (DNASE1) ELISA Kit | KTE71229

Mouse Deoxyribonuclease-1 (DNASE1) ELISA Kit | KTE71229 | Gentaur UK, US & Europe Distribution

Application: This Mouse Deoxyribonuclease-1 (DNASE1) ELISA Kit employs a two-site sandwich ELISA to quantitate DNASE1 in samples. An antibody specific for DNASE1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDNASE1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DNASE1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DNASE1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Deoxyribonuclease-1 microplate
• Mouse Deoxyribonuclease-1 standard
• Mouse Deoxyribonuclease-1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Deoxyribonuclease-1 (DNASE1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse DNASE1. No significant cross-reactivity or interference between Mouse DNASE1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: DNase I is a nuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide, yielding 5'-phosphate terminated polynucleotides with a free hydroxyl group on position 3', on average producing tetranucleotides. It acts on single-stranded DNA, double-stranded DNA, and chromatin. It has been suggested to be one of the deoxyribonucleases responsible for DNA fragmentation during apoptosis.DNase I binds to the cytoskeletal protein actin. It binds actin monomers with very high (sub-nanomolar) affinity and actin polymers with lower affinity. The function of this interaction is unclear. However since actin-bound DNase I is enzymatically inactive, the DNase-actin complex might be a storage form of DNase I that prevents damage of the genetic information.

Alternative Names: DNASE1; DKFZp686H0155; DNL1; DRNI; FLJ38093; FLJ44902; DNase I; lysosomal; Dornase alfa; human urine deoxyribonuclease I

Search name: DNASE1; DKFZp686H0155; DNL1; DRNI; FLJ38093; FLJ44902; DNase I; lysosomal; Dornase alfa; human urine deoxyribonuclease I

Tag: DNASE1