Mouse Diamine oxidase (DAO) ELISA Kit | KTE70533

Mouse Diamine oxidase (DAO) ELISA Kit | KTE70533 | Gentaur UK, US & Europe Distribution

Application: This Mouse Diamine oxidase (DAO) ELISA Kit employs a two-site sandwich ELISA to quantitate ABP1 in samples. An antibody specific for ABP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyABP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ABP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ABP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Diamine oxidase microplate
• Mouse Diamine oxidase standard
• Mouse Diamine oxidase detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Diamine oxidase (DAO) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse ABP1. No significant cross-reactivity or interference between Mouse ABP1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: On the basis of its primary structure, the amiloride-binding protein (EC 1.4.3.6) is 713 amino acids long, with a 19-amino acid signal peptide. Expressed in cultured cells, the mRNA yields a glycoprotein that binds amiloride and amiloride analogs with affinities similar to the amiloride receptor associated with the apical Na+ channel in pig kidney membranes and is immunoprecipitated with monoclonal antibodies raised against pig kidney amiloride-binding protein. Barbry et al. (1990) pointed out that amiloride-sensitive Na+ channels are also present in airway epithelia, where they play an important role in fluid secretion. Amiloride inhibits the excessive absorption of Na+ and liquid that takes place in airway epithelia of patients with cystic fibrosis, and amiloride aerosol therapy has been tried for the treatment of lung disease in CF.

Alternative Names: ABP1; ABP; AOC1; DAO; DAO1; KAO; amiloride binding protein 1; amiloride-binding protein; amiloride-binding protein-1; amiloride-sensitive amine oxidase; diamine oxidase; histaminase; kidney amine oxidase

Search name: ABP1; ABP; AOC1; DAO; DAO1; KAO; amiloride binding protein 1; amiloride-binding protein; amiloride-binding protein-1; amiloride-sensitive amine oxidase; diamine oxidase; histaminase; kidney amine oxidase

Tag: ABP1