Mouse Dipeptidyl peptidase 8 (DPP8) ELISA Kit | KTE71225

Mouse Dipeptidyl peptidase 8 (DPP8) ELISA Kit | KTE71225 | Gentaur UK, US & Europe Distribution

Application: This Mouse Dipeptidyl peptidase 8 (DPP8) ELISA Kit employs a two-site sandwich ELISA to quantitate DPP8 in samples. An antibody specific for DPP8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDPP8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DPP8 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DPP8 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Dipeptidyl peptidase 8 microplate
• Mouse Dipeptidyl peptidase 8 standard
• Mouse Dipeptidyl peptidase 8 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Dipeptidyl peptidase 8 (DPP8) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse DPP8. No significant cross-reactivity or interference between Mouse DPP8 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Dipeptidyl peptidase 8 is member of the peptidase S9B family, a small family of dipeptidyl peptidases that are able to cleave peptide substrates at a prolyl bond. The encoded protein shares similarity with dipeptidyl peptidase IV in that it is ubiquitously expressed, and hydrolyzes the same substrates. These similarities suggest that, like dipeptidyl peptidase IV, this protein may play a role in T-cell activation and immune function. Alternatively spliced transcript variants encoding different isoforms have been described.The deduced 882-amino acid DPP8 protein shares about 27% sequence identity with DPP4 and FAP; unlike these proteins, however, DPP8 has no transmembrane domain and no N- or O-linked glycosylation sites.

Alternative Names: DPP8; DP8; DPRP1; FLJ14920; FLJ20283; MGC26191; MSTP141; dipeptidyl peptidase 8; dipeptidyl peptidase IV-related protein-1; dipeptidyl peptidase VIII; dipeptidylpeptidase 8; prolyl dipeptidase DPP8

Search name: DPP8; DP8; DPRP1; FLJ14920; FLJ20283; MGC26191; MSTP141; dipeptidyl peptidase 8; dipeptidyl peptidase IV-related protein-1; dipeptidyl peptidase VIII; dipeptidylpeptidase 8; prolyl dipeptidase DPP8

Tag: DPP8