Mouse Dixin (DIXDC1) ELISA Kit | KTE71269

Mouse Dixin (DIXDC1) ELISA Kit | KTE71269 | Gentaur UK, US & Europe Distribution

Application: This Mouse Dixin (DIXDC1) ELISA Kit employs a two-site sandwich ELISA to quantitate DIXDC1 in samples. An antibody specific for DIXDC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDIXDC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DIXDC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DIXDC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Dixin microplate
• Mouse Dixin standard
• Mouse Dixin detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Dixin (DIXDC1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse DIXDC1. No significant cross-reactivity or interference between Mouse DIXDC1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: The 3-prime UTR of the cDNA contains an Alu element, and the deduced protein contains 493 amino acids. RT-PCR ELISA detected high expression in adult whole brain and in several specific brain regions. Intermediate expression was detected in all other tissues and brain regions examined. The deduced 472-amino acid DIXDC1 protein has a central myosin tail homology (MTH) region similar to those found in MYH1 and MYH2, as well as a C-terminal DIX domain. Within the MTH region, DIXDC1 contains 2 tyrosine phosphorylation sites and a leucine zipper motif. DIXDC1 was predicted to be a cytoplasmic protein.In contrast with human DIXDC1, the MTH region of mouse Dixdc1 has only 1 tyrosine phosphorylation site and no leucine zipper motif.

Alternative Names: DIXDC1; CCD1; KIAA1735;

Search name: DIXDC1; CCD1; KIAA1735;

Tag: DIXDC1