Mouse DLAT (Dihydrolipoyl Transacetylase) ELISA Kit | ELK2372

Mouse DLAT (Dihydrolipoyl Transacetylase) ELISA Kit | ELK2372 | Gentaur UK, US & Europe Distribution

Alternative Names: DLTA; PDC-E2; PBC; M2 antigen complex 70 kDa subunit; Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; Pyruvate dehydrogenase complex E2

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.62 ng/mL

Standard: 100 ng/mL

Detection Range: 1.57-100 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase;Infection immunity;Hepatology;Gastroenterology;Autoimmunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dihydrolipoyl Transacetylase (DLAT) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dihydrolipoyl Transacetylase (DLAT) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dihydrolipoyl Transacetylase (DLAT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dihydrolipoyl Transacetylase (DLAT) in the samples is then determined by comparing the OD of the samples to the standard curve.