Mouse DNA-directed RNA polymerase I subunit RPA1 (POLR1A) ELISA Kit | KTE71447

Mouse DNA-directed RNA polymerase I subunit RPA1 (POLR1A) ELISA Kit | KTE71447 | Gentaur UK, US & Europe Distribution

Application: This Mouse DNA-directed RNA polymerase I subunit RPA1 (POLR1A) ELISA Kit employs a two-site sandwich ELISA to quantitate POLR1A in samples. An antibody specific for POLR1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPOLR1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for POLR1A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of POLR1A bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse DNA-directed RNA polymerase I subunit RPA1 microplate
• Mouse DNA-directed RNA polymerase I subunit RPA1 standard
• Mouse DNA-directed RNA polymerase I subunit RPA1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse DNA-directed RNA polymerase I subunit RPA1 (POLR1A) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse POLR1A. No significant cross-reactivity or interference between Mouse POLR1A and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: POLR1A belongs to the RNA polymerase beta' chain family.DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol I. A bridging helix emanates from RPA1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol I by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition

Alternative Names: POLR1A; A190; DKFZp586M0122; FLJ21915; MGC87965; RPA1; RPA194; RPO1-4; RPO14; DNA-directed RNA polymerase I A; DNA-directed RNA polymerase I largest subunit; DNA-directed RNA polymerase I subunit A1

Search name: POLR1A; A190; DKFZp586M0122; FLJ21915; MGC87965; RPA1; RPA194; RPO1-4; RPO14; DNA-directed RNA polymerase I A; DNA-directed RNA polymerase I largest subunit; DNA-directed RNA polymerase I subunit A1

Tag: POLR1A